Brno University of Technology

4 ARTICLES PUBLISHED IN JoVE

Chemistry

Aerosol-assisted Chemical Vapor Deposition of Metal Oxide Structures: Zinc Oxide Rods

Stella Vallejos^1,2, Naděžda Pizúrová³, Jan Čechal⁴, Isabel Gràcia¹, Carles Cané¹

¹Instituto de Microelectrónica de Barcelona (IMB-CNM, CSIC), ²SIX Research Centre, Brno University of Technology, ³Institute of Physics of Material, Academy of Science of Czech Republic, ⁴Institute of Physical Engineering and Central European Institute of Technology, Brno University of Technology

Columnar zinc oxide structures in the form of rods are synthesized via aerosol-assisted chemical vapor deposition without the use of pre-deposited catalyst-seed particles. This method is scalable and compatible with various substrates based on either silicon, quartz or polymers.

Chemistry

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Yazan Haddad^1,2, Simona Dostalova^1,2, Jiri Kudr^1,2, Ondrej Zitka^1,2, Zbynek Heger^1,2, Vojtech Adam^1,2

¹Department of Chemistry and Biochemistry, Mendel University in Brno, ²Central European Institute of Technology, Brno University of Technology

This protocol describes the synthesis of magnetic particles and evaluation of their DNA-binding properties via dynamic and electrophoretic light scattering. This method focuses on monitoring changes in particle size, their polydispersity and zeta potential of particle surface which play major role in binding of materials such as DNA.

Neuroscience

Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo

Hana Uhlirova^1,2, Peifang Tian^3,4, Kıvılcım Kılıç^3,5, Martin Thunemann¹, Vishnu B. Sridhar⁶, Radim Chmelik^2,7, Hauke Bartsch¹, Anders M. Dale^1,3, Anna Devor^1,3,8, Payam A. Saisan³

¹Department of Radiology, University of California, San Diego, ²Central European Institute of Technology, Brno University of Technology, ³Department of Neurosciences, University of California, San Diego, ⁴Department of Physics, John Carroll University, ⁵Department of Biomedical Engineering, Boston University, ⁶Bioengineering Undergraduate Program, University of California, San Diego, ⁷Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, ⁸Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School

A graphical user interface for exploring and sharing a database of optogenetically-induced vascular responses in mouse somatosensory cortex in vivo measured by 2-photon microscopy is presented. It allows browsing the data, criteria-based selection, averaging, localization of measurements within a 3D volume of vasculature and exporting the data.

Engineering

Fabrication of Magnetic Nanostructures on Silicon Nitride Membranes for Magnetic Vortex Studies Using Transmission Microscopy Techniques

Meena Dhankhar¹, Marek Vaňatka¹, Michal Urbanek¹

¹CEITEC BUT, Brno University of Technology

A protocol for the fabrication of magnetic micro- and nanostructures with spin configurations forming magnetic vortices suitable for transmission electron microscopy (TEM) and magnetic transmission x-ray microscopy (MTXM) studies is presented.