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Montana State University

6 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
Matthew P. Taylor 1,2, Radomir Kratchmarov 2, Lynn W. Enquist 2
1Department of Immunology and Infectious Diseases, Montana State University, 2Department of Molecular Biology, Princeton University

Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.

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Immunology and Infection

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
Timothy R. Borgogna 1, Adrian Sanchez-Gonzalez 2, Kelly Gorham 2, Jovanka M. Voyich 1
1Department of Microbiology and Immunology, Montana State University, 2University Communications, Montana State University

Here, we present methods to improve secondary bacterial pneumonia studies by providing a non-invasive route of instillation into the lower respiratory tract followed by pathogen recovery and transcript analysis. These procedures are reproducible and can be performed without specialized equipment such as cannulas, guide wires, or fiber optic cables.

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Immunology and Infection

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Jennifer G. Dankoff 1, Kyler B. Pallister 1, Fermin E. Guerra 1, Alexander J. Parks 1, Kelly Gorham 2, Saul Mastandrea 2, Jovanka M. Voyich 1, Tyler K. Nygaard 1
1Department of Microbiology and Immunology, Montana State University, 2University Communications, Montana State University

This protocol describes a method for the purification of polymorphonuclear leukocytes from whole human blood and two distinct assays that quantify the cytotoxicity of Staphylococcus aureus against these important innate immune cells.

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Biology

Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research
Kelli Buckingham-Meyer 1, Lindsey A. Miller 1, Albert E. Parker 1, Diane K. Walker 1, Paul Sturman 1, Ian Novak 1, Darla M. Goeres 1
1Center for Biofilm Engineering, Montana State University

This paper details methods that demonstrate three common biofilm harvesting and disaggregation techniques on two surface types, ruggedness testing of a harvesting method and minimum information to consider when choosing and optimizing harvesting and disaggregation techniques to increase reproducibility.

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Biology

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
Robert C. Moseley 1, Sophia Campione 1, Bree Cummins 2, Francis Motta 3, Steven B. Haase 1
1Department of Biology, Duke University, 2Department of Mathematical Sciences, Montana State University, 3Department of Mathematical Sciences, Florida Atlantic University

The Inherent Dynamics Visualizer is an interactive visualization package that connects to a gene regulatory network inference tool for enhanced, streamlined generation of functional network models. The visualizer can be used to make more informed decisions for parameterizing the inference tool, thus increasing confidence in the resulting models.

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Medicine

Profiling Luminal pH in Three-Dimensional Gastrointestinal Organoids Using Microelectrodes
Katrina Lyon 1, Rama Bansil 2, Diane Bimczok 1
1Department of Microbiology & Cell Biology, Montana State University, 2Department of Physics, Boston University

The present protocol describes pH measurements in human tissue-derived gastric organoids using microelectrodes for spatiotemporal characterization of intraluminal physiology.

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