JoVE Logo

Sign In

Rigshospitalet

3 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
Changsi Cai 1, Stefan A. Zambach 1, Jonas C. Fordsmann 1, Micael Lønstrup 1, Kirsten J. Thomsen 1, Aske G. K. Jensen 1, Martin Lauritzen 1,2
1Department of Neuroscience, Faculty of Medicine and Health Science, University of Copenhagen, 2Department of Clinical Neurophysiology, Rigshospitalet

We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.

image

Immunology and Infection

Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay
Maria del Pilar Quintana 1, Nsoh Godwin Anabire 1,2,3, Lars Hviid 1,4
1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, 3Department of Immunology, Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases, Rigshospitalet

The overall goal of this protocol is to provide instruction on how to measure the capacity of antibodies present in sera or plasma of individuals, naturally exposed to Plasmodium falciparum infection, to opsonize and induce phagocytosis of the parasite-infected erythrocytes (IEs).

image

Neuroscience

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model
Jesper Peter Bömers 1,2, Sara Ellinor Johansson 2, Lars Edvinsson 2,4, Tiit Illimar Mathiesen 1,3,5, Kristian Agmund Haanes 2
1Department of Neurosurgery, Rigshospitalet, 2Department of Clinical Experimental Research, Glostrup Research Institute, Rigshospitalet, 3Department of Clinical Medicine, University of Copenhagen, 4Department of Clinical Sciences, Division of Experimental Vascular Research, Lund University, 5Department of Clinical Neuroscience, Karolinska Institutet

Subarachnoid hemorrhage continues to carry a high burden of mortality and morbidity in man. To facilitate further research into the condition and its pathophysiology, a pre-chiasmatic, single injection model is presented.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved