Kansai Medical University

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Preparing Activated Platelet-Rich Plasma for Culturing Human Adipose-Derived Stem Cells

Fangyuan Lai^1,2, Natsuko Kakudo¹, Naoki Morimoto¹, Yuanyuan Ma¹, Kenji Kusumoto¹

¹Department of Plastic and Reconstructive Surgery, Kansai Medical University, ²Department of Plastic Surgery, Zhejiang Provincial People's Hospital

In this report, we describe a double-spin method for preparing activated platelet-rich plasma (PRP). Using autologous thrombin, human adipose-derived stem cells (hASCs) were cultured. Activated PRP was shown to promote proliferation of hASCs.

Bioengineering

A Novel Cell Injection Method with Minimum Invasion

Fumiyuki Hattori¹

¹Innovative Regenerative Medicine, Graduate School of Medicine, Kansai Medical University

This method eliminates any major invasion during cell injections caused by the cell suspension solution.