Norwegian Veterinary Institute

1 ARTICLES PUBLISHED IN JoVE

Genetics

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Snorre Gulla¹, Saima Nasrin Mohammad¹, Duncan John Colquhoun^1,2

¹Fish Health Research Group, Norwegian Veterinary Institute, ²University of Bergen

The Multi-Locus Variable-number tandem-repeat Analysis (MLVA) assay presented here enables inexpensive, robust and portable high-resolution genotyping of the fish-pathogenic bacterium Yersinia ruckeri. Starting from pure cultures, the assay employs multiplex PCR and capillary electrophoresis to produce ten-loci MLVA profiles for downstream applications.