Experimental Glaucoma Induced by Ocular Injection of Magnetic MicrospheresShannon Bunker 1, Joanna Holeniewska 1, Sauparnika Vijay 2, Annegret Dahlmann-Noor 2,3, Peng Khaw 2,4, Yin-Shan Ng 5, David Shima 1,6, Richard Foxton 1
1Ocular Biology and Therapeutics, University College London Institute of Ophthalmology, 2University College London Institue of Ophthalmology, 3Moorfields Eye Hospital, 4NIHR Biomedical Research Centre, Moorfields Eye Hospital, 5Schepens Eye Research Institute, Harvard Medical School, 6Hoffman-La Roche
We present a method for inducing elevated intraocular pressure (IOP), by injecting magnetic microspheres into the rat eye, to model glaucoma. This leads to strong pressure rises, and extensive neuronal death. This protocol is easy to perform, does not require repeat injections, and produces stable long-lasting IOP rises.
