DUNDALK INSTITUTE OF TECHNOLOGY

2 ARTICLES PUBLISHED IN JoVE

Biology

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca²⁺ Signaling In Situ

Bernard T. Drumm¹, Grant W. Hennig², Salah A. Baker¹, Kenton M. Sanders¹

¹Department of Physiology and Cell Biology, University of Nevada Reno School of Medicine, ²Department of Pharmacology, The Robert Larner, M.D. College of Medicine, University of Vermont

Genetically encoded Ca²⁺ indicators (GECIs) have radically changed how in situ Ca²⁺ imaging is performed. To maximize data recovery from such recordings, appropriate analysis of Ca²⁺ signals is required. The protocols in this paper facilitate the quantification of Ca²⁺ signals recorded in situ using spatiotemporal mapping and particle-based analysis.

Biology

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Nathan Grainger^1,2, Kenton M. Sanders¹, Bernard T. Drumm^1,3

¹Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, ²Department of Physiology & Membrane Biology, University of California School of Medicine, ³Department of Life & Health Sciences, Dundalk Institute of Technology

The goal of this protocol is to isolate intact pacemaker regions of the mouse renal pelvis using vibratome sectioning. These sections can then be used for in situ Ca²⁺ imaging to elucidate Ca²⁺ transient properties of pacemaker cells and other interstitial cells in vibratome slices.