University of Aix-Marseille

3 ARTICLES PUBLISHED IN JoVE

Biology

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

Harold N. Baker¹, Robin Murphy¹, Erica Lopez¹, Carlos Garcia^1,2

¹Chemistry Research and Development, Luminex Corporation, ²Global Marketing, Luminex Corporation

An ELISA can be easily converted to a Luminex xMAP assay and, through the benefits of multiplexing, several antibodies can be screened simultaneously to identify an optimum antibody pair, resulting in increased sensitivity and dynamic range, while reducing assay cost.

Medicine

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Keith K. Fenrich¹, Pascal Weber¹, Genevieve Rougon^1,2, Franck Debarbieux^1,2

¹Developmental Biology Institute of Marseille-Luminy (IBDML), Aix Marseille University, ²Campus de la Timone, European Research Center for Medical Imaging (CERIMED)

We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon microscopy.

Immunology and Infection

Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics

Sreekanth Dasari¹, Pascal Weber¹, Camélia Makhloufi¹, Erica Lopez², Claire-Lise Forestier¹

¹INSERM 1095, URMITE, University of Aix-Marseille, ²U.F.R de Médecine, University of Aix-Marseille

This article reports on a detailed method for the dynamic measurement and quantification of blood flow velocity within individual blood vessels of the mouse liver vasculature using intravital microscopy imaging in combination with a specific methodology for image acquisition and analysis.