Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect PestsYao-Chia Liu 1, Nian-Tong Ni 1, Ju-Chun Chang 1, Yi-Hsuan Li 1, Mi Rong Lee 2, Jae Su Kim 2,3, Yu-Shin Nai 1
1Department of Entomology, National Chung Hsing University, 2Department of Agricultural biology, Jeonbuk National University, 3Department of Agricultural Convergence Technology, Jeonbuk National University
Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.
