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King Abdullah University of Science and Technology

6 ARTICLES PUBLISHED IN JoVE

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Chemistry

Synthesis of Non-uniformly Pr-doped SrTiO3 Ceramics and Their Thermoelectric Properties
Arash Mehdizadeh Dehkordi 1, Sriparna Bhattacharya 2, Taghi Darroudi 3, Xiaoyu Zeng 2, Husam N. Alshareef 4, Terry M. Tritt 1,2
1Department of Materials Science and Engineering, Clemson University, 2Department of Physics and Astronomy, Clemson University, 3Electron Microscope Facility, Clemson University, 4Materials Science and Engineering, King Abdullah University of Science and Technology

A protocol for the synthesis and processing of polycrystalline SrTiO3 ceramics doped non-uniformly with Pr is presented along with the investigation of their thermoelectric properties.

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Bioengineering

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Gabriel E. Büchel 1,2, Brandon Carney 1,3, Jun Tang 1, Brian M. Zeglis 1,3, Jörg Eppinger 2, Thomas Reiner 1,4
1Department of Radiology, Memorial Sloan Kettering Cancer Center, 2KAUST Catalysis Center, King Abdullah University of Science and Technology, 3Department of Chemistry, Hunter College, and PhD Program in Chemistry, Graduate Center of City University of New York, 4Department of Radiology, Weill Cornell Medical College

This protocol describes a new intraoperative imaging technique that uses a ruthenium complex as a source of chemiluminescent light emission, thereby producing high signal-to-noise ratios during in vivo imaging. Intraoperative imaging is an expanding field that could revolutionize the way that surgical procedures are performed.

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JoVE Core

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
Corrado Calì 1, Kalpana Kare 1, Marco Agus 2, Maria Fernanda Veloz Castillo 1, Daniya Boges 1, Markus Hadwiger 2, Pierre Magistretti 1
1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, 2Visual Computing Center, King Abdullah University of Science and Technology

The described pipeline is designed for the segmentation of electron microscopy datasets larger than gigabytes, to extract whole-cell morphologies. Once the cells are reconstructed in 3D, customized software designed around individual needs can be used to perform a qualitative and quantitative analysis directly in 3D, also using virtual reality to overcome view occlusion.

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JoVE Core

Iron Nanowire Fabrication by Nano-Porous Anodized Aluminum and its Characterization
Niketan S. Patel 1, David Lago-Cachón 1, Hanan Mohammed 1, Julián A. Moreno 1, Jürgen Kosel 1
1Sensing, Magnetism and Microsystems Group, Computer Electrical and Mathematical Science and Engineering Division, King Abdullah University of Science and Technology

In this work, we describe a protocol to fabricate iron nanowires, including the formation of the porous alumina membrane that is used as the template, electrodeposition into templates using electrolyte solution, and the release of the nanowires into the solution.

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Bioengineering

Biofunctionalization of Magnetic Nanomaterials
Nouf A. Alsharif 1, Jasmeen S. Merzaban 2, Jürgen Kosel 1,2
1Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, 2Division of Computer, Electrical and Mathematical Sciences and Engineering, King Abdullah University of Science and Technology

In this work, we provide a protocol to biofunctionalize magnetic nanomaterials with antibodies for specific cell targeting. As examples, we utilize iron nanowires to target cancer cells.

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Bioengineering

Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix
Rosario Perez-Pedroza 1,2, Manola Moretti 1,2,3, Charlotte A. E. Hauser 1,2,3
1Laboratory for Nanomedicine, Biological & Environmental Science & Engineering, King Abdullah University of Science and Technology, 2Computational Bioscience Research Center, King Abdullah University of Science and Technology, 3Red Sea Research Center, King Abdullah University of Science and Technology

This protocol aims to evaluate biofunctional self-assembling peptides for cell adhesion, organoid morphology, and gene expression by immunostaining. We will use a colorectal cancer cell line to provide a cost-effective way of obtaining organoids for intensive testing.

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