Hospital of the University of Pennsylvania

3 ARTICLES PUBLISHED IN JoVE

Medicine

Jennifer E. Douglas^1,2, Corrine J. Mansfield², Charles J. Arayata², Beverly J. Cowart², Lauren R. Colquitt², Ivy W. Maina^1,2, Mariel T. Blasetti³, Noam A. Cohen³, Danielle R. Reed²

¹Perelman School of Medicine at the University of Pennsylvania, ²Monell Chemical Senses Center, ³Department of Otorhinolaryngology-Head and Neck Surgery, Division of Rhinology, Hospital of the University of Pennsylvania

This protocol measures human taste responses and includes a brief anatomical assessment, a short taste test, and a validation method using the subject's reported sensation and taste receptor genotype.

Cancer Research

Molecular and Immunologic Techniques in a Genetically Engineered Mouse Model of Gastrointestinal Stromal Tumor

Andrew D. Tieniber¹, Andrew N. Hanna¹, Kevin Do¹, Laura Wang¹, Ferdinando Rossi¹, Ronald P. DeMatteo¹

¹Department of Surgery, Hospital of the University of Pennsylvania

The goal of this manuscript is to describe the Kit^V558Δ/+ mouse model and techniques for successful dissection and processing of mouse specimens.

Bioengineering

In Vitro Thrombosis Test for Ventricular Assist Devices

Mansur Zhussupbekov¹, Scott Stelick¹, Rugveda Thanneeru², Shivbaskar Rajesh², Salim E. Olia³, Harvey S. Borovetz², James F. Antaki¹

¹Meinig School of Biomedical Engineering, Cornell University, ²Department of Bioengineering, University of Pittsburgh, ³Department of Cardiovascular Surgery, Hospital of the University of Pennsylvania

We present a benchtop protocol to induce thrombosis in ventricular assist devices (VAD) within a recirculating test platform. This method serves to identify thrombogenic hotspots in the blood flow path and can help improve thromboresistance ahead of preclinical testing in animal models.