Johannes Gutenberg University Medical Center

2 ARTICLES PUBLISHED IN JoVE

Virus-induced Gene Knockdown in the CA3 and Subsequent Spatial Relocation Test to Assess CA3-dependent Cognitive Function

Mercè Masana^1,2, Ulrich Schmitt¹, Konstantin Radyushkin³, Andrés Uribe-Mariño², Marianne B Müller^1,2

¹Department of Psychiatry and Psychotherapy & Focus Program Translational Neuroscience (FTN), Johannes Gutenberg University Medical Center, ²Max-Planck Institute of Psychiatry, ³Focus Program Translational Neurosciences (FTN), Johannes Gutenberg University of Mainz

Hippocampal cognitive function can be modulated by locally manipulating gene expression. The spatial object relocation test is a short and robust spatial memory test. We combined this test with virus-induced manipulation of gene expression in the CA3 region to assess the impact of the target gene in shaping cognitive performance.

Behavior

Social Threat-Safety Test Uncovers Psychosocial Stress-Related Phenotypes

Sarah Ayash¹, Marianne Müller^1,2, Ulrich Schmitt¹

¹Leibniz-Institute for Resilience Research, ²Translational Psychiatry, Department of Psychiatry and Psychotherapy, Johannes Gutenberg University Medical Center

The social threat-safety test allows a simultaneous assessment of social avoidance development as a measurement of aversive conditioned learning and social threat-safety discrimination ability, both utilized to identify stress-susceptible and stress-resilient individuals within a single group of chronically socially defeated male mice.