Johannes Gutenberg University of Mainz

1 ARTICLES PUBLISHED IN JoVE

Virus-induced Gene Knockdown in the CA3 and Subsequent Spatial Relocation Test to Assess CA3-dependent Cognitive Function

Mercè Masana^1,2, Ulrich Schmitt¹, Konstantin Radyushkin³, Andrés Uribe-Mariño², Marianne B Müller^1,2

¹Department of Psychiatry and Psychotherapy & Focus Program Translational Neuroscience (FTN), Johannes Gutenberg University Medical Center, ²Max-Planck Institute of Psychiatry, ³Focus Program Translational Neurosciences (FTN), Johannes Gutenberg University of Mainz

Hippocampal cognitive function can be modulated by locally manipulating gene expression. The spatial object relocation test is a short and robust spatial memory test. We combined this test with virus-induced manipulation of gene expression in the CA3 region to assess the impact of the target gene in shaping cognitive performance.