Antwerp University Hospital

6 ARTICLES PUBLISHED IN JoVE

Medicine

Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain

¹Translational Neurosciences, University of Antwerp, ²Multidisciplinary Pain Center, Antwerp University Hospital

This manuscript describes a rodent model to study trigeminal neuropathic pain. The methods described include surgical procedures to perform a chronic constriction injury of the rat’s infraorbital nerve and the post-surgical behavioral tests to measure the changes in spontaneous and evoked behavior that are indicative of persistent pain and allodynia.

Neuroscience

In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments

Sara Nullens¹, Annemie Deiteren², Wen Jiang³, Christopher Keating⁴, Hannah Ceuleers¹, Sven Francque⁵, David Grundy³, Joris G. De Man¹, Benedicte Y. De Winter¹

¹Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, ²Visceral Pain Group, Discipline of Medicine, University of Adelaide, ³Department of Biomedical Sciences, University of Sheffield, ⁴Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, ⁵Department of Gastroenterology and Hepatology, Antwerp University Hospital

Mesenteric afferent nerves convey information from the gastrointestinal tract towards the brain regarding normal homeostasis as well as pathophysiology. Gastrointestinal afferent nerve activity can be assessed by mounting isolated intestinal segments with attached afferent nerves into an organ bath, isolating the nerve, and assessing basal as well as stimulated activity.

Education

Objective Nociceptive Assessment in Ventilated ICU Patients: A Feasibility Study Using Pupillometry and the Nociceptive Flexion Reflex

Davina Wildemeersch¹, Jens Gios², Philippe G. Jorens³, Guy H. Hans²

¹Multidisciplinary Pain Center, Anesthesiology and Critical Care Medicine, Antwerp University Hospital (UZA), University of Antwerp (UA), ²Multidisciplinary Pain Center (PCT), Antwerp University Hospital (UZA), ³Critical Care Medicine, Antwerp University Hospital (UZA), University of Antwerp (UA)

Pain assessment in anesthetized patients who cannot communicate with the outside world in any way remains challenging despite the development of innovative objective pain evaluation tools. In this project, the pupillary dilation reflex and the nociception flexion reflex are assessed in critically ill, mechanically ventilated adult patients.

Medicine

Endolymphatic Duct Blockage as a Surgical Treatment Option for Ménière's Disease

Annejet Alida Schenck^1,2, Issam Saliba³, Josephina Maria Kruyt⁴, Peter Paul van Benthem², Hendrikus Maria Blom^1,2,5

¹Department of Otorhinolaryngology, Haga Hospital, ²Department of Otorhinolaryngology, Leiden University Medical Centre, ³Division of Otolaryngology-Head and Neck Surgery, Montreal University Hospital Center (CHUM), University of Montreal, ⁴Department of Otorhinolaryngology, Bergman Clinics, ⁵Department of Otorhinolaryngology, Antwerp University Hospital

Endolymphatic duct blockage is a relatively new surgical method for patients suffering from Ménière's disease. Following a regular mastoidectomy, the endolymphatic duct is identified and ligated using a regular titanium hemoclip. The effectiveness of this procedure is currently being assessed in a randomized trial.

Medicine

Performing Repeated Intraoperative Impedance Telemetry Measurements during Cochlear Implantation

Nora M. Weiss^1,2,3,4,5, Stefan Hans⁶, Martin Wozniak⁶, Aline Föger⁶, Stefan Dazert¹, Vincent Van Rompaey^2,7, Paul Van de Heyning^2,7, Joachim Schmutzhard⁸, Angelika Dierker⁶

¹Department of Otorhinolaryngology-Head and Neck Surgery, Ruhr-University Bochum, St. Elisabeth-Hospital Bochum, ²Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, ³International Graduate School of Neuroscience (IGSN), Ruhr-University Bochum, ⁴Technical University of Munich, ⁵Department of Otorhinolaryngology, TUM School of Medicine, Klinikum rechts der Isar, ⁶MED-EL, ⁷Department of Otorhinolaryngology and Head & Neck Surgery, Antwerp University Hospital, ⁸Department of Otorhinolaryngology-Head and Neck Surgery, Medical University Innsbruck

Here we present a protocol to conduct repeated impedance telemetry measurements during cochlear implantation (CI). They may allow conclusions on the electrode's and implant's function. Repeated impedance measurements enable objective feedback on whether the electrode is positioned inside the perilymph or outside the inner ear.

Neuroscience

Chronic Constriction Injury of the Distal Infraorbital Nerve (DIoN-CCI) in Mice to Study Trigeminal Neuropathic Pain

Kristof R. Deseure^1,2, Guy H. Hans^1,2

¹Antwerp Surgical Training, Anatomy and Research Centre, Algology (pain research), University of Antwerp, ²Multidisciplinary Pain Center, Antwerp University Hospital

Chronic constriction injury of the distal infraorbital nerve in mice induces changes in spontaneous behavior (increased face grooming activity) and nocifensive behavior in response to tactile stimulation (hyperresponsiveness to von Frey hair stimulation) that are signs of ongoing pain and allodynia and serves as a model for trigeminal neuropathic pain.