JoVE Logo

Sign In

Rush University Medical Center

5 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
Zachary B. Davis 1, Jeffrey P. Ward 1, Edward Barker 1
1Department of Immunology and Microbiology, Rush University Medical Center

Cytotoxicity assays to measure natural killer cell lytic responses to HIV-infected cells is limited by the purity of the target cells. We demonstrate here the isolation of a highly purified population of HIV-1 infected primary T-cell blasts by taking advantage of HIV-1 s ability to down-modulate CD4.

image

Biology

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
Ki Ho Park 1, Noah Weisleder 2, Jingsong Zhou 3, Kristyn Gumpper 1, Xinyu Zhou 1, Pu Duann 4, Jianjie Ma 1, Pei-Hui Lin 1
1Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 2Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, 3Department of Molecular Biophysics and Physiology, Rush University Medical Center, 4Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center

Described here is a method to directly measure calcium sparks, the elementary units of Ca2+ release from sarcoplasmic reticulum in intact skeletal muscle fibers. This method utilizes osmotic-stress-mediated triggering of Ca2+ release from ryanodine receptor in isolated muscle fibers. The dynamics and homeostatic capacity of intracellular Ca2+ signaling can be employed to assess muscle function in health and disease.

image

Medicine

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure
Erik Schadde 1,2,3, Martin Hertl 2, Stefan Breitenstein 3, Beatrice Beck-Schimmer 1,4, Martin Schläpfer 1,4
1Institute of Physiology - Center for Integrative Human Physiology, University of Zurich, 2Department of Surgery, Rush University Medical Center, 3Department of Surgery, Cantonal Hospital Winterthur, 4Institute of Anesthesiology, University and University Hospital Zurich

Inducing rapid liver hypertrophy using Associating Liver Partition and Portal vein ligation for a Staged hepatectomy (ALPPS) has been proposed for resection of borderline resectable liver tumors. This model may elucidate mechanisms involved in rapid hypertrophy and allows testing of drugs that promote or block the acceleration of regeneration.

image

Medicine

Estimation of Nephron Number in Whole Kidney using the Acid Maceration Method
Sarah M. Peterson 1, Xuexiang Wang 2, Ashley C. Johnson 1, Ian D. Coate 1, Michael R. Garrett 1,3, Sean P. Didion 1,4
1Department of Pharmacology and Toxicology, The University of Mississippi Medical Center, 2Department of Medicine, Division of Nephrology, Rush University Medical Center, 3Department of Medicine, Division of Nephrology, The University of Mississippi Medical Center, 4Department of Neurology, The University of Mississippi Medical Center

Estimates of whole kidney nephron number are important clinically and experimentally, as there is an inverse association between nephron number and an enhanced risk of renal and cardiovascular disease.Herein, the use of the acid maceration method, which provides fast and reliable estimates of whole kidney nephron number, is demonstrated.

image

Medicine

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
Cristina L. Fhied *1, Imad Tarhoni *1, David Gerard 1, Grant M. Lewin 1, Hita Moudgalya 1, Jeffrey R. Schneider 2, Jeffrey A. Borgia 1,3
1Department of Anatomy & Cell Biology, Rush University Medical Center, 2Department of Microbial Pathogens and Immunity, Rush University Medical Center, 3Department of Pathology, Rush University Medical Center

This article describes a convenient method for monitoring dynamic changes in antibody titers for two immunoglobulin isotypes concurrently (either IgA, IgM, or IgG) resulting from an immune response to SARS-CoV-2 infection or vaccination. This 'Multianalyte Covid-19 Immune Response Panel' employs three indirect immunoassays built on coded microspheres that are read using a flow-based multiplex reader with 'dual-channel' capability.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved