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Anhui Agricultural University

4 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats
Shihai Jiang *1, Xiaoyou Yi *3, Yuansen Luo 2, Dongjie Yu 1, Yuangao Liu 1, Fei Zhang 1,4, Lei Zhu 2, Kun Wang 1
1Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 2Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 3Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, 4Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University

Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.

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Biochemistry

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves
Weiting Jiao *1,2, Guoqin Ge *1, Rimao Hua 2, Jun Sun 1, Yeyun Li 1, Ruyan Hou 1
1State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Province Key Lab of Analysis and Detection for Food Safety, 2School of Resource & Environment, Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province

This work presents a protocol for establishing a cell suspension culture derived from tea (Camellia sinensis L.) leaves that can be used to study the metabolism of external compounds that can be taken up by the whole plant, such as insecticides.

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Environment

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples
Weiting Jiao 1,2, Tingting Shen 2, Luyao Wang 1, Lei Zhu 1,3, Qing X. Li 4, Chen Wang 3, Hongping Chen 3, Rimao Hua 1, Xiangwei Wu 1
1Key Laboratory of Agri-food Safety of Anhui Province, School of Resource & Environment, Anhui Agricultural University, 2State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, 3Key Laboratory of Tea Quality and Safety & Risk Assessment, Tea Research Institute, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, 4Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa

The present protocol describes the contamination of pyrrolizidine alkaloids (PAs) in tea samples from PA-producing weeds in tea gardens.

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Chemistry

Tea Aroma Analysis Based on Solvent-Assisted Flavor Evaporation Enrichment
Zhihui Feng 1, Xiaogen Yang 2, Chun Zou 1, Junfeng Yin 1
1Tea Research Institute Chinese Academy of Agricultural Sciences, 2State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University

Presented here is a method for enriching and analyzing the volatile components of tea extracts using solvent-assisted flavor evaporation and solvent extraction followed by gas chromatography-mass spectrometry, which can be applied to all types of tea samples.

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