Thiruvalluvar University

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Tzu-Cheng Sung^*1, Hsing-Fen Li^*1, Akon Higuchi^1,2, Qing-Dong Ling^3,4, Jia-Sin Yang¹, Yeh-Chia Tseng¹, Chih-Hsien Pan Pan¹, Abdullah A. Alarfaj², Murugan A. Munusamy², Suresh Kumar⁵, Shih-Tien Hsu⁶, Kadarkarai Murugan^7,8

¹Department of Chemical and Materials Engineering, National Central University, ²Department of Botany and Microbiology, King Saud University, ³Cathay Medical Research Institute, Cathay General Hospital, ⁴Graduate Institute of Systems Biology and Bioinformatics, National Central University, ⁵Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, ⁶Department of Internal Medicine, Taiwan Landseed Hospital, ⁷Department of Zoology, Bharathiar University, ⁸Thiruvalluvar University

A protocol is presented to prepare polyvinyl alcohol-co-itaconic acid hydrogels with varying stiffness, which were grafted with and without oligopeptides, to investigate the effect of the stiffness of biomaterials on the differentiation and proliferation of stem cells. The stiffness of the hydrogels was controlled by the crosslinking time.

Cancer Research

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Saswati Das^1,2, Raveen Stephen Stallon Illangeswaran^1,2, Smitha Ijee^2,3, Sanjay Kumar^2,3, Shaji Ramachandran Velayudhan^1,2,3, Poonkuzhali Balasubramanian^1,2,3

¹Department of Clinical Haematology, Christian Medical College, ²Department of Biotechnology, Thiruvalluvar University, ³Centre for Stem Cell Research, Christian Medical College

High-throughput RNA interference (RNAi) screening using a pool of lentiviral shRNAs can be a tool to detect therapeutically relevant synthetic lethal targets in malignancies. We provide a pooled shRNA screening approach to investigate the epigenetic effectors in acute myeloid leukemia (AML).

Biology

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

Vigneshwaran Venkatesan^*1,2, Abisha Crystal Christopher^*1,3, Karthik V. Karuppusamy^1,2, Prathibha Babu^1,2, Manoj Kumar K. Alagiri^1,2, Saravanabhavan Thangavel¹

¹Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru, Christian Medical College campus, ²Manipal Academy of Higher Education, ³Thiruvalluvar University

The present protocol describes an optimized hematopoietic stem and progenitor cell (HSPC) culture procedure for the robust engraftment of gene-edited cells in vivo.