An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout MiceFlorencia S. Fontana Estevez *1, Verónica Miksztowicz *1, Ignacio M. Seropián 1,2, Pablo Cassaglia 3, Romina Bustos 1, Vanessa Touceda 1, Tomas Cianciulli 4, Verónica Cassanova 5, Celina Morales 3, Germán E. González 1
1Laboratorio de Patología Cardiovascular Experimental e Hipertensión Arterial, Instituto de Investigaciones Biomédicas, Universidad Católica Argentina-CONICET, 2Servicio de Hemodinamia y Cardiología Intervencionista, Hospital Italiano de Buenos Aires, 3Instituto de Fisiopatologia Cardiovascular, Facultad de Medicina, Universidad de Buenos Aires, 4División Cardiología, Hospital General de Agudos "Dr. Cosme Argerich,", 5Facultad de Veterinaria, Universidad de Buenos Aires
Here, we describe an experimental model of myocardial infarction, an echocardiography procedure to study cardiac remodeling and function, and procedures for quantifying fibrosis and hypertrophy in picrosirius red-stained and rhodamine-stained sections, as well as the infarct size and expansion index in slices stained with Masson's trichrome.
