Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern FormationsMasaya Hagiwara 1, Rina Nobata 2, Tomohiro Kawahara 3
1NanoSquare Research Institute, Osaka Prefecture University, 2Department of Bioscience and Informatics, Osaka Prefecture University, 3Department of Biological Functions Engineering, Kyushu Institute of Technology
We present a procedure for controlling the initial cell cluster shape in a 3D extracellular matrix to obtain a repeatable pattern formation. A cubic device containing two different hydrogels is employed to achieve multi-directional imaging for tissue pattern formation.