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Murray State University

3 ARTICLES PUBLISHED IN JoVE

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Biology

An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos
Ming Gao 1, Patrick McCluskey 1, Sudan N. Loganathan 1, Alexey L. Arkov 1
1Department of Biological Sciences, Murray State University

Multi-component protein complexes play crucial roles during cellular function and development. Here we describe a method used to isolate native protein complexes from Drosophila embryos after in vivo crosslinking followed by purification of the crosslinked complexes for subsequent structure-function analysis.

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Developmental Biology

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae
Dena R. Hammond-Weinberger 1, Gary T. ZeRuth 1
1Department of Biological Sciences, Murray State University

Here, we present a protocol for fluorescent antibody-mediated detection of proteins in whole preparations of zebrafish embryos and larvae.

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JoVE Core

Analysis and Specification of Starch Granule Size Distributions
Ming Gao 1, Mahta Moussavi 1, Deland Myers 1
1Cooperative Agriculture Research Center, College of Agriculture and Human Science, Prairie View A&M University

Presented here is a procedure for reproducible and statistically valid determinations of starch granule size distributions, and for specifying the determined granule lognormal size distributions using a two-parameter multiplicative form. It is applicable to all granule sizing analyses of gram-scale starch samples for plant and food science research.  

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