National Taiwan University Hospital and College of Medicine

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Yu-Jen Chen^1,2, Hui-Yu Liu², Yun-Tsui Chang², Ying-Hung Cheng², Harry J. Mersmann², Wen-Hung Kuo³, Shih-Torng Ding^1,2

¹Institute of Biotechnology, National Taiwan University, ²Department of Animal Science and Technology, National Taiwan University, ³Department of Surgery, National Taiwan University Hospital and College of Medicine

This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.

Medicine

Ultrasonographic Assessment During Cardiopulmonary Resuscitation

Wan-Ching Lien¹, Chih-Heng Chang², Wei-Tien Chang^1,3, Wen-Jone Chen^1,3

¹Department of Emergency Medicine, National Taiwan University Hospital and College of Medicine, ²Department of Emergency Medicine, National Taiwan University Hospital Chin-Shan Branch, ³Cardiovascular Division, Department of Internal Medicine, National Taiwan University Hospital and College of Medicine

Here, we present a US-CAB (Ultrasound, Circulation/Airway/Breathing) protocol for use during cardiopulmonary resuscitation (CPR). US-C evaluates the subxiphoid view of the heart and inferior vena cava. After intubation, tracheal US (US-A) and lung US (US-B) help confirm endotracheal intubation and proper ventilation.