JoVE | Peer Reviewed Scientific Video Journal

Immunology and Infection

Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster

Shuo Yang^*1,2,3, Yaya Zhao^*1,2, Junjing Yu⁴, Zhiqin Fan², Si-tang Gong¹, Hong Tang^1,2, Lei Pan^1,2,3

¹The Joint Center for Infection and Immunity, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, ²CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, ³CAS Key Laboratory of Infection and Immunity (CASKLII), Institute of Biophysics, Chinese Academy of Sciences, ⁴Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences

This protocol describes how to establish viral infection in vivo in Drosophila melanogaster using the nano-injection method and basic techniques to analyze virus-host interaction.

Medicine

Protocol and Guidelines for Point-of-Care Lung Ultrasound in Diagnosing Neonatal Pulmonary Diseases Based on International Expert Consensus

Jing Liu^1,2, Roberto Copetti³, Erich Sorantin⁴, Jovan Lovrenski⁵, Javier Rodriguez-Fanjul⁶, Dalibor Kurepa⁷, Xing Feng⁸, Luigi Cattaross⁹, Huayan Zhang^10,11, Misun Hwang¹², Tsu F. Yeh^13,14, Yisrael Lipener⁷, Abhay Lodha¹⁵, Jia-Qin Wang¹⁶, Hai-Ying Cao^2,17, Cai-Bao Hu^2,18, Guo-Rong Lyu¹⁹, Xin-Ru Qiu^1,2, Li-Qun Jia²⁰, Xiao-Man Wang²⁰, Xiao-Ling Ren^1,2, Jiu-Ye Guo^1,2, Yue-Qiao Gao^1,2, Jian-Jun Li^1,2, Ying Liu^1,2, Wei Fu^1,2, Yan Wang²¹, Zu-Lin Lu^1,2, Hua-Wei Wang⁸, Li-Li Shang²²

¹Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, ²The Neonatal Lung Ultrasound Training Base, Chinese College of Critical Ultrasound, ³Emergency Department, Cattinara University Hospital, ⁴Division of Pediatric Radiology, Department of Radiology, Medical University Graz, ⁵Faculty of Medicine, University of Novi Sad, Radiology Department, Institute for Children and Adolescents Health Care of Vojvodina, ⁶Pediatric Intensive Care Unit, Pediatric Service Hospital Joan XXIII Tarragona, University Rovira i Virgil, ⁷Division of Neonatal-Perinatal Medicine, Cohen Children's Medical Center, ⁸Department of Neonatology, Children's Hospital of Soochow University, ⁹Department of Neonatology, Udine University Hospital, ¹⁰Center for Newborn Care, Guangzhou Women and Children's Medical Center, ¹¹Division of Neonatology, Children's Hospital of Philadelphia, ¹²Department of Radiology, Children's Hospital of Philadelphia, ¹³Division of Neonatology and NICU, Cook County Children's Hospital, University of Illinois, ¹⁴Division of Neonatology, Department of Pediatrics, Taipei Medical University, ¹⁵Department of Pediatrics and Community Health Sciences, University of Calgary, ¹⁶Department of Pediatrics, The Third Affiliated Hospital of Xinxiang Medical University, ¹⁷Department of Ultrasound, GE Healthcare, ¹⁸Intensive Care Unit, Zhejiang Hospital, ¹⁹Collaborative Innovation Center for Maternal and Infant Health Service Application Technology, Quanzhou Medical College, ²⁰Department of Ultrasound, Beijing Children's Hospital Affiliated with Capital Medical University, ²¹Department of Neonatology and NICU, Tai'an City Central Hospital of Shandong Province, ²²Department of Intensive Care Unit, The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine

Lung ultrasound is a noninvasive and valuable tool for bedside evaluation of neonatal lung diseases. However, a relative lack of reference standards, protocols and guidelines may limit its application. Here, we aim to develop a standardized neonatal lung ultrasound diagnostic protocol to be used in clinical decision-making.

JoVE Core

International Expert Consensus and Recommendations for Neonatal Pneumothorax Ultrasound Diagnosis and Ultrasound-guided Thoracentesis Procedure

Jing Liu^1,2, Dalibor Kurepa³, Francesco Feletti^4,5, Almudena Alonso-Ojembarrena⁶, Jovan Lovrenski⁷, Roberto Copetti⁸, Erich Sorantin⁹, Javier Rodriguez-Fanjul¹⁰, Karishma Katti³, Andrea Aliverti⁴, Huayan Zhang^11,12, Misun Hwang¹³, Tsu F. Yeh¹⁴, Cai-Bao Hu¹⁵, Xing Feng¹⁶, Ru-Xin Qiu^1,2, Jing-Han Chi¹⁷, Li-Li Shang¹⁸, Guo-Rong Lyu¹⁹, Shao-Zheng He²⁰, Yan-Fen Chai²¹, Zhan-Jun Qiu²², Hai-Ying Cao^2,23, Yue-Qiao Gao^1,2, Xiao-Ling Ren^1,2, Guo Guo^1,24, Li Zhang^1,2, Ying Liu^1,2, Wei Fu^1,2, Zu-Lin Lu^1,2, Hong-Lei Li^1,2

¹Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, ²The National Neonatal Lung Ultrasound Training Base, ³Division of Neonatal-Perinatal Medicine, Cohen Children's Medical Center, ⁴Department of Electronics, Information and Bioengineering, Politecnico di Milano, ⁵Dipartimento di Diagnostica per Immagini, Ausl della Romagna, S. Maria delle Croci Hospital, ⁶Neonatal Intensive Care Unit, Puerta del Mar University Hospital, ⁷Faculty of Medicine, University of Novi Sad, Serbia, Institute for Children and Adolescents Health Care of Vojvodina, ⁸Emergency Department, University Hospital of Cattinara, ⁹Division of Pediatric Radiology, Department of Radiology, Medical University Graz, ¹⁰Pediatric Intensive Care Unit, Pediatric Service Hospital Joan XXIII Tarragona, University Rovira i Virgil, ¹¹Center for Newborn Care, Guangzhou Women and Children's Medical Center, ¹²Division of Neonatology, Children's Hospital of Philadelphia, ¹³Section of Neonatal Imaging, Department of Radiology, Children's Hospital of Philadelphia, ¹⁴Maternal Child Health Research institute, Taipei Medical University and China Medical University, ¹⁵Intensive Care Unit, Zhejiang Hospital, ¹⁶Department of Neonatology, Children's Hospital of Soochow University, ¹⁷Department of Neonatology and NICU, Bayi Children's Hospital Affiliated to the Seventh Medical Center of Chinese PLA General Hospital, ¹⁸Intensive Care Unit, Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, ¹⁹Collaborative Innovation Center for Maternal and Infant Health Service Application Technology, Quanzhou Medical College, ²⁰Department of Ultrasound, Second Affiliated Hospital of Fujian Medical University, ²¹Department of Emergency Medicine, Tianjin Medical University General Hospital, ²²Department of Emergency and Critical Care Medicine, Affiliated Hospital of Traditional Chinese Medicine, ²³Department of Ultrasound, GE Healthcare, ²⁴The Neonatal Intensive Care Unit, Fifth Medical Center of Chinese PLA General Hospital

Pneumothorax is a common emergency and critical disease in newborn infants that needs rapid, clear diagnosis and timely treatment. Diagnosis and treatment based on chest X-rays are associated with delayed management and radiation damage. Lung ultrasound (US) provides useful guidance for rapid, accurate diagnosis and the precise thoracentesis of pneumothorax.

Guangzhou Women and Children's medical center

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Medicine

JoVE Core