Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Birgit Perner¹, Danny Schnerwitzki¹, Michael Graf^1,3, Christoph Englert^1,2

¹Molecular Genetics, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), ²Faculty of Biology and Pharmacy, Friedrich Schiller University of Jena, ³Carl Zeiss Microscopy GmbH

The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.