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Zhejiang Chinese Medical University

12 ARTICLES PUBLISHED IN JoVE

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Medicine

Application of Granger Causality Analysis of the Directed Functional Connection in Alzheimer's Disease and Mild Cognitive Impairment
Mei Wang 1, Zhengluan Liao 2, Dewang Mao 3, Qi Zhang 1, Yumei Li 3, Enyan Yu 2, Zhongxiang Ding 3
1Zhejiang Chinese Medical University, 2Department of Psychiatry, Zhejiang Provincial People's Hospital, 3Department of Radiology, Zhejiang Provincial People's Hospital

Based on resting-state functional magnetic resonance imaging with Granger causality analysis, we investigated the alterations in the directed functional connectivity between the posterior cingulate cortex and whole brain in patients with Alzheimer's Disease (AD), patients with Mild Cognitive Impairment (MCI), and healthy controls.

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Biochemistry

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer
Zhihui Xu *1, Yiyan Song *1, Huijun Jiang 2, Yan Kong 3, Xiaoming Li 3, Jin Chen 1, Yuan Wu 4
1School of Public Health, Nanjing Medical University, 2School of Pharmacy, Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University

This protocol describes a general strategy to regenerate commercial arrayed gold microelectrodes equipped for a label-free cell analyzer aimed at saving on the high running costs ofmicrochip-based assays. The regeneration process includes trypsin digestion, rinsing with ethanol and water, and a spinning step, which enables repeated usage of microchips.

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Behavior

An Automated T-maze Based Apparatus and Protocol for Analyzing Delay- and Effort-based Decision Making in Free Moving Rodents
Qi Zhang 1,2, Yuki Kobayashi 1, Hiromichi Goto 1, Shigeyoshi Itohara 1
1Laboratory of Behavioral Genetics, Center for Brain Science, RIKEN, 2Faculty of Human Science, University of Tsukuba

This article introduces an automated T-maze apparatus that we invented, and a protocol based on this apparatus for analyzing delay-based decision making and effort-based decision making in free moving rodents.

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Behavior

Chronic Post-Ischemia Pain Model for Complex Regional Pain Syndrome Type-I in Rats
Qimiao Hu *1, Xiaoli Zheng *1, Ruixiang Chen 1, Boyu Liu 1, Yan Tai 2, Xiaomei Shao 1, Jianqiao Fang 1, Boyi Liu 1
1Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Key Laboratory of Acupuncture and Neurology of Zhejiang Province, 2Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University

Provided here is a protocol that details steps to establish an animal model of chronic post-ischemia pain (CPIP). This is a well-recognized model mimicking human complex regional pain syndrome type-I. Mechanical and thermal hypersensitivities are further evaluated, as well as capsaicin-induced nocifensive behaviors observed in the CPIP rat model.

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Medicine

Osteoarthritis Pain Model Induced by Intra-Articular Injection of Mono-Iodoacetate in Rats
Jiaan Xu 1,2, Li Yan 1,2, Bo Yan 1,2, Li Zhou 1, Peijian Tong 1, Letian Shan 1
1The First Affiliated Hospital, Zhejiang Chinese Medical University, 2College of Pharmaceutical Science, Zhejiang Chinese Medical University

This study describes the method of intra-articular injection of mono-iodoacetate in rats and discusses the resulting pain-related behaviors and histopathological changes, which provide references for future applications.

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Immunology and Infection

Visualization of Macrophage Lytic Cell Death During Mycobacterial Infection in Zebrafish Embryos via Intravital Microscopy
Liangfei Niu 1, Cong Wang 1, Kaile Zhang 1,2, Miaomiao Kang 1,2, Rui Liang 1, Xiaonan Zhang 1, Bo Yan 1
1Shanghai Public Health Clinical Center, Fudan University, 2School of Life Sciences, Bengbu Medical College

This protocol describes a technique for visualizing macrophage behavior and death in embryonic zebrafish during Mycobacterium marinum infection. Steps for the preparation of bacteria, infection of the embryos, and intravital microscopy are included. This technique may be applied to the observation of cellular behavior and death in similar scenarios involving infection or sterile inflammation.

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Developmental Biology

Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts
Yujie Huang *1, Yizhou Tao *1, Chang Cai 1, Jin Chen 1, Cheng Ji 1, Stanley Aniagu 2, Yan Jiang 1, Tao Chen 1
1School of Public Health, Soochow University, 2Toxicology, Risk Assessment and Research Division, Texas Commission on Environmental Quality

This protocol uses an immunofluorescence assay to detect PM2.5-induced DNA damage in the dissected hearts of zebrafish embryos.

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Medicine

Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method
Xiaoli Jin *1, Yang Li *2, Xuanwei Chen 1, Jin Chen 1, Jian Xu 1
1School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 2School of Basic Medical Sciences, Fudan University

Here we present a protocol for the isolation of BMMs from SD rats, called the secondary adherence method.

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Biochemistry

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
Xinghui Song 1, Hao Shen 2, Yanwei Li 3, Yueting Xing 3, Jiajia Wang 3, Chun Guo 3, Yingying Huang 3, Jin Chen 2
1Central Laboratory of Women’s Hospital, Zhejiang University School of Medicine, 2School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 3Core Facilities, Zhejiang University School of Medicine

This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters.

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Medicine

Development and Evaluation of a Rat Model of Full-Thickness Cartilage Defects
Haiyan Zhang *1, Ronghua Bao *2, Jiaan Xu 3, Yanzhi Ge 4, Zuxiang Chen 1, Mengqiang Fan 1, Guangping Yu 5, Li Zhou 1, Le Guo 3, Letian Shan 1, Hangxing Bao 1
1The First Affiliated Hospital, Zhejiang Chinese Medical University, 2Fuyang Orthopaedics and Traumatology Affiliated Hospital, Zhejiang Chinese Medical University, 3College of Pharmaceutical Science, Zhejiang Chinese Medical University, 4Affiliated Zhejiang Hospital, Zhejiang University School of Medicine, 5Xianju County Hospital of Chinese Medicine

This protocol establishes a full-thickness cartilage defects (FTCD) model by drilling holes in the femoral trochlear groove of rats and measuring the subsequent pain behavior and histopathological changes.

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Medicine

Manual Therapy for a Chronic Non-Specific Low Back Pain Rat Model
Shuang-wei Hong *1, Kai-zheng Wang *1, Xing-chen Zhou 1, Hui-jie Hu 1, Yu Tian 1, Hua-zhi Huang 1, Zhen Liu 1, Zhi-zhen Lv 1,2, Li-jiang Lv 1,2
1The Third School of Clinical Medicine, School of Rehabilitation Medicine, Zhejiang Chinese Medical University, 2Research Institute of Tuina (Spinal disease), Zhejiang Chinese Medical University

This study proposes a standardized procedure for manual therapy on chronic low back pain model rats, which will be of reference value for future experimental research on manual therapy.

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Medicine

Improved Thoracoscopic-Assisted Surgery for the Treatment of Metastatic Thoracic Vertebral Tumors
Xiao Long *1, Xiaodi Zou *1,2, Xiang Tong 1, Dongdong Yu 1, Hui Li 1, Yuxin Chang 1, Bin Chen 1, Hui Lu 1
1Department of Orthopedics, The First Affiliated Hospital, Zhejiang University, 2Department of Orthopedics, The Second Affiliated Hospital, Zhejiang Chinese Medical University

Here, we present a protocol to demonstrate an improved thoracoscopic-assisted surgery for the treatment of metastatic thoracic vertebral tumors.

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