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Western Michigan University

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation
Cynthia A. Gossman 1, David M. Linn 2, Cindy Linn 1
1Department of Biological Sciences, Western Michigan University, 2Department of Biomedical Sciences, Grand Valley State University

Glaucoma is characterized by damage to retinal ganglion cells. Inducing glaucoma in animal models can provide insight into the study of this disease. Here, we outline a procedure that induces loss of RGCs in an in vivo rat model and demonstrates the preparation of whole-mount retinas for analysis.

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Developmental Biology

Surgical Ablation Assay for Studying Eye Regeneration in Planarians
Jacob M. Morton *1, Marwa A. Saad *1, Wendy S. Beane 1
1Department of Biological Sciences, Western Michigan University

This protocol shows how to consistently excise planarian eyes (optic cups) without disturbing surrounding tissues. Using an insulin needle and syringe, either one or both eyes can be ablated to facilitate investigations into the mechanisms regulating eye regeneration, the evolution of visual regeneration, and the neural basis of light-induced behavior.

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Neuroscience

Social Isolation Model: A Noninvasive Rodent Model of Stress and Anxiety
Saki Watanabe 1, Alzahra Al Omran 1, Amy S. Shao 2, Jing Liang 1
1Titus Family Department of Clinical Pharmacy, School of Pharmacy, University of Southern California, 2Homer Stryker M.D. School of Medicine, Western Michigan University

Presented here is a social isolation (SI)-induced anxiety mouse model that utilizes wild type C56BL/6J mice to induce stress and anxiety-like behavior with minimal handling and no invasive procedures. This model reflects modern life patterns of social isolation and is ideal for studying anxiety and related disorders.

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