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Chongqing University of Science and Technology

5 ARTICLES PUBLISHED IN JoVE

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Biology

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Tyler M. Bauman 1,2, Emily A. Ricke 2, Sally A. Drew 3, Wei Huang 3,4, William A. Ricke 2,4
1Division of Urologic Surgery, Washington University in St. Louis School of Medicine, 2Department of Urology, University of Wisconsin School of Medicine and Public Health, 3Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, 4O’Brien Urology Research Center, University of Wisconsin School of Medicine and Public Health

Immunohistochemistry is a powerful lab technique for evaluating protein localization and expression within tissues. Current semi-automated methods for quantitation introduce subjectivity and often create irreproducible results. Herein, we describe methods for multiplexed immunohistochemistry and objective quantitation of protein expression and co-localization using multispectral imaging.

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Engineering

Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion
Shihui Liu 1, Kang Du 1, Kejun Wen 1, Wei Huang 2, Farshad Amini 1, Lin Li 3
1Department of Civil & Environmental Engineering, Jackson State University, 2School of Civil Engineering and Architecture, Chongqing University of Science and Technology, 3Department of Civil & Architectural Engineering, Tennessee State University

Here, microbially induced calcite precipitation (MICP) technology is presented to improve soil properties by immersion.

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Medicine

Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits
Robert A. Honkanen 1, Liqun Huang 2,3, Wei Huang 1, Basil Rigas 2,4
1Department of Ophthalmology, Stony Brook University, 2Department of Medicine, Stony Brook University, 3Medicon Pharmaceuticals, Inc, 4Department of Preventive Medicine, Stony Brook University

A novel approach is presented to induce chronic dry eye disease in rabbits by surgically removing all orbital lacrimal glands. This method, distinct from those previously reported, produces a stable, reproducible model of aqueous deficient dry eye well suited to study tear physiology and pathophysiology and ocular therapeutics.

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Medicine

A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo
Yihai Liu 1, Wenfeng Zhang 1, Tingting Hu 1, Jie Ni 1, Biao Xu 1, Wei Huang 1
1Department of Cardiology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University

Described is a protocol to establish a Doxorubicin-induced dilated cardiomyopathy (DCM) model in mice via long-term intraperitoneal injection of Doxorubicin.

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Medicine

Real-Time Dynamic Navigation System for the Precise Quad-Zygomatic Implant Placement in a Patient with a Severely Atrophic Maxilla
Yihan Shen *1, Qinggang Dai *1, Baoxin Tao *1, Wei Huang 2, Feng Wang 2, Kengliang Lan 1, Yuanyuan Sun 1, Xiaowan Ling 1, Lijun Yan 1, Yueping Wang 1, Yiqun Wu 1
1The 2nd Dental Center, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, 2Department of Dental Implantology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology

Here, we present a protocol to achieve precise quad-zygomatic implant placement in patients with severely atrophic maxilla using a real-time dynamic navigation system.

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