Guangdong University of Technology

5 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination

¹Department of Bioengineering, Bourns College of Engineering, University of California, Riverside

A novel method involving quantitative analysis of FRET (Förster Resonance Energy Transfer) signals is described for studying enzyme kinetics. K_M and k_cat were obtained for the hydrolysis of the catalytic domain of SENP1 (SUMO/Sentrin specific protease 1) to pre-SUMO1 (Small Ubiquitin-like MOdifier). The general principles of this quantitative-FRET-based protease kinetic study can be applied to other proteases.

Developmental Biology

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Caramai N. Kamei¹, Yan Liu^1,2, Iain A. Drummond^1,3

¹Nephrology Division, Department of Medicine, Massachusetts General Hospital, ²Basic Sciences Division, Fred Hutchinson Cancer Research Center, ³Department of Genetics, Harvard Medical School

Here we present a reliable method to study adult kidney regeneration by inducing acute kidney injury by gentamicin injection. We show that injury is dependent on gentamicin dosage and environmental temperature using in situ hybridization to label lhx1a+ developing new nephrons.

JoVE Journal

Synthesis of a Thiol Building Block for the Crystallization of a Semiconducting Gyroidal Metal-sulfur Framework

Yonghe He¹, Jiahong Huang¹, Jun He¹, Zhengtao Xu²

¹School of Chemical Engineering and Light Industry, Guangdong University of Technology, ²Department of Chemistry, City University of Hong Kong

Here, we present a one-pot, transition-metal-free synthesis of thiols and thioesters from aromatic halides and sodium thiomethoxide, followed by the preparation of single crystals of a metal-dithiolene network using thiol species generated in situ from the more stable and tractable thioester.

JoVE Journal

Chemoselective Preparation of 1-Iodoalkynes, 1,2-Diiodoalkenes, and 1,1,2-Triiodoalkenes Based on the Oxidative Iodination of Terminal Alkynes

Youzhi Li^*1, Daya Huang^*1, Ju Huang¹, Yan Liu¹, Keiji Maruoka^1,2

¹School of Chemical Engineering and Light Industry, Guangdong University of Technology, ²Department of Chemistry, Graduate School of Science, Kyoto University

Herein, detailed protocols for the oxidative iodination of terminal alkynes using hypervalent-iodine reagents are presented, which chemoselectively afford 1-iodoalkynes, 1,2-diiodoalkenes, and 1,1,2-triiodoalkenes.

Behavior

A Behavioral Test Battery for the Repeated Assessment of Motor Skills, Mood, and Cognition in Mice

Ran You^1,2,3, Yan Liu³, Raymond Chuen-Chung Chang³

¹Nanjing Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, ²Nanjing Key Laboratory of Pediatrics, Nanjing Medical University, ³Laboratory of Neurodegenerative Disease, Li Ka Shing Faculty of Medicine, University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region (HK SAR)

A comprehensive behavioral test battery of motor skills, mood—including social interaction, depression, and anxiety—and cognition is designed for the repeated assessment of neurodegeneration-related behavioral changes in mice.