Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic CellsJingna Yang 1, Fangyuan Kang 1, Anqi Wei 1, Wenyan Lu 1, Xinfu Zhang 1, Liqiang Han 1
1College of Veterinary Medicine, Henan Agricultural University
The present protocol describes how to use oil red O to dye lipid droplets (LDs), calculate the size and number of LDs in a fatty acid-induced fatty hepatocyte model, and use BODIPY 493/503 to observe the process of small LDs fusing into large LDs by live cell imaging.
