Jiangsu University of Science and Technology

4 ARTICLES PUBLISHED IN JoVE

Bioengineering

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Avani V. Pisapati¹, Yi Wang^*2, Megan E. Blauch^*3, Nathan J. Wittenberg³, Xuanhong Cheng^1,2, X. Frank Zhang^1,4

¹Department of Bioengineering, Lehigh University, ²Department of Materials Science and Engineering, Lehigh University, ³Department of Chemistry, Lehigh University, ⁴Department of Mechanical Engineering and Mechanics, Lehigh University

We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.

Immunology and Infection

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β₂-Microglobulin

Di Zhang^*1,2, Kefang Liu^*3,4, Dan Lu^*2,5, Pengyan Wang^1,2, Qingxu Zhang^1,2, Peipei Liu², Yingze Zhao², Yan Chai⁴, Jianxin Lyu¹, Jianxun Qi⁴, William J. Liu^1,2

¹School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, ²NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, ³Faculty of Health Sciences, University of Macau, ⁴CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, ⁵Savaid Medical School, University of Chinese Academy of Sciences

The protocol describes experimental methods to obtain stable major histocompatibility complex (MHC) class I through potential β₂-microglobulin (β₂m) substitutions from different species. The structural comparison of MHC I stabilized by homologous and heterologous β₂m were investigated.

Developmental Biology

In Ovo Intravascular Injection in Chicken Embryos

Kai Jin^1,2, Jing Zhou^1,2, Gaoyuan Wu^1,2, Ziyi Lian^1,2, Zongyi Zhao^1,2, Shujian Zhou^1,2, Chen Chen^1,2, Hongyan Sun^1,2, Yingjie Niu^1,2, Qishenng Zuo^1,2, Yani Zhang^1,2, Jiuzhou Song³, Guohong Chen^1,2, Bichun Li^1,2,4

¹Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, ²Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, ³Animal & Avian Sciences, University of Maryland, College Park, ⁴College of Biotechnology, Jiangsu University of Science and Technology

The overall goal of this paper is to describe how to perform in ovo intracellular injection of exogenous materials into chicken embryos. This approach is very useful to study the developmental biology of chicken embryos.

Behavior

Usability Evaluation of Augmented Reality: A Neuro-Information-Systems Study

Jun Wu^*1, Di Zhang^*1, Tao Liu^2,3,4, Helen Hong Yang⁵, Yi Wang¹, Huili Yao¹, Shinan Zhao¹

¹Economics and Management School, Jiangsu University of Science and Technology, ²School of Health, Fujian Medical University, ³School of Management, Shanghai University, ⁴School of Management, Zhejiang University, ⁵Business School, La Trobe University

This study presents an experimental paradigm for a usability test combining subjective and objective evaluations. The objective evaluation adopted Neuro-Information-Systems (NeuroIS) methods, and the subjective evaluation adopted a usability questionnaire and a NASA-Task Load Index (NASA-TLX) scale.