North China Electric Power University

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS

Taylor R. Fore¹, Audrey A. Ojwang¹, Margaret L. Warner¹, Xinyun Peng¹, Rudolf A. Bohm^1,2, William P. Welch¹, Lindsey K. Goodnight¹, Hong Bao¹, Bing Zhang¹

¹Department of Zoology, University of Oklahoma - Norman, ²Department of Biology, Brandeis University

We describe a Flippase-induced intersectional Gal80/Gal4 repression (FINGR) method, allowing tissue-specific FLP to determine Gal80 expression patterns. Wherever Gal4 and FLP overlap, Gal4 expression is turned on (Gal80 flipped out) or off (Gal80 flipped in). The FINGR method is versatile for clonal analysis and neural circuit mapping.

JoVE Core

Enhanced Electron Injection and Exciton Confinement for Pure Blue Quantum-Dot Light-Emitting Diodes by Introducing Partially Oxidized Aluminum Cathode

Zhibin Wang¹, Tai Cheng¹, Fuzhi Wang¹, Yiming Bai¹, Xingming Bian¹, Bing Zhang¹, Tasawar Hayat^2,3, Ahmed Alsaedi³, Zhan'ao Tan¹

¹State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources, North China Electric Power University, ²Department of Mathematics, Quaid-I-Azam University, ³NAAM Research Group, Faculty of Science, King Abdulaziz University

A protocol is presented for fabricating high-performance, pure blue ZnCdS/ZnS-based quantum dots light-emitting diodes by employing an autoxidized aluminum cathode.

Biology

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

Tianming Wang¹, Xu Chen¹, Ke Xu¹, Bing Zhang¹, Dexiang Huang¹, Jingwen Yang¹

¹National Engineering Research Center of Marine Facilities Aquaculture, Marine Science College, Zhejiang Ocean University

This protocol provides an easy-to-handle method to culture the intestinal cells from sea cucumber Apostichopus japonicus and is compatible with a variety of widely available tissue samples from marine organisms including Echinodermata, Mollusca, and Crustacea.

Genetics

Assisted Selection of Biomarkers by Linear Discriminant Analysis Effect Size (LEfSe) in Microbiome Data

Fang Chang^*1, Shishi He^*2, Chenyuan Dang³

¹Marine Resources Research Centre, Tianjin Research Institute for Water Transport Engineering, M.O.T., ²Key Laboratory of Regional Energy Systems Optimization, Ministry of Education, College of Environmental Science and Engineering, North China Electric Power University, ³School of Environmental Science and Engineering, Huazhong University of Science and Technology

LEfSe (LDA Effect Size) is a tool for high-dimensional biomarker mining to identify genomic features (such as genes, pathways, and taxonomies) that significantly characterize two or more groups in microbiome data.