Zhejiang Gongshang University

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Uri Rozovski¹, Inbal Hazan-Halevy², George Calin³, David Harris⁴, Ping Li⁴, Zhiming Liu⁴, Michael J. Keating⁴, Zeev Estrov⁴

¹Division of Hematology, Davidoff Cancer Center, Rabin Medical Center, ²The Center of Nanoscience and Nanotechnology, Tel Aviv University, ³Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, ⁴Department of Leukemia, The University of Texas MD Anderson Cancer Center

Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.

JoVE Core

Applicability Analysis of Assessment Methods for Morphological Parameters of Corroded Steel Bars

Dawang Li^1,2, Ping Li^1,2, Yingang Du³, Ren Wei^1,2

¹Department of Civil Engineering, Shenzhen University, ²Guangdong Province Key Laboratory of Durability for Marine Civil Engineering, Shenzhen University, ³Department of Engineering and the Built Environment, Anglia Ruskin University

This paper measures the geometry and the amount of corrosion of a steel bar using different methods: mass loss, calipers, drainage measurements, 3D scanning, and X-ray micro-computed tomography (XCT).

Immunology and Infection

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Yunfei Hu¹, Huahai Chen¹, Ping Li², Baiyuan Li¹, Linyan Cao¹, Changhui Zhao¹, Qing Gu², Yeshi Yin¹

¹Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, ²College of Food and Biological Engineering, Zhejiang Gongshang University

Described here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.