China Medical University

5 ARTICLES PUBLISHED IN JoVE

Behavior

Manipulation of Epileptiform Electrocorticograms (ECoGs) and Sleep in Rats and Mice by Acupuncture

Pei-Lu Yi^*1, Shuo-Bin Jou^*2, Yi-Jou Wu³, Fang-Chia Chang^3,4,5

¹Department of Sports, Health & Leisure, College of Tourism, Leisure and Sports, Aletheia University, Tainan Campus, ²Department of Neurology, Mackay Memorial Hospital and Mackay Medical College, ³Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, ⁴Graduate Institute of Brain & Mind Sciences, College of Medicine, National Taiwan University, ⁵Graduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical University

This paper demonstrates the performance of acupuncture, epilepsy models, and the analysis of sleep in rodents. The acupuncture procedure and the identification of acupoints are described. Pilocarpine or pentylenetetrazol (PTZ) is used to induce epilepsy. Electrocorticogram (ECoG), electromyogram (EMG), brain temperature, and locomotor activity recordings are employed for sleep analysis.

Medicine

Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery

Shao-Chieh Chiu¹, Shih-Ting Hsu¹, Chiun-Wei Huang¹, Wu-Chung Shen², Shin-Lei Peng²

¹Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital, ²Department of Biomedical Imaging and Radiological Science, China Medical University

The overall goal of this procedure is to measure the blood flow in the rat common carotid artery by using noninvasive phase contrast magnetic resonance imaging.

Cancer Research

Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

Chi-Hao Hsiao^1,2,3, Ya-Hsu Chiu⁴, Shao-Chih Chiu^4,5, Der-Yang Cho^4,6,7, Liang-Ming Lee^1,2, Yu-Ching Wen^1,2, Jia-Jhe Li⁴, Ping-Hsiao Shih⁴

¹Department of Urology, Wan Fang Hospital, ²Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, ³Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, ⁴Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, ⁵Graduate Institute of Biomedical Sciences, China Medical University, ⁶Graduate Institute of Immunology, China Medical University, ⁷Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital

Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3⁺CD56⁺ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.

Medicine

Fu's Subcutaneous Needling for Knee Osteoarthritis Pain

Po-En Chiu^*1,2, Zhonghua Fu^*3,4, Jian Sun^4,5,6, Guan-Wei Jian^7,8, Te-Mao Li⁹, Li-Wei Chou^10,11,12

We present a protocol for using Fu's subcutaneous needling for knee osteoarthritis pain, which combines swaying movement and reperfusion approach techniques. This protocol has great potential for future applications in myofascial pain treatment and could enhance Fu's subcutaneous needling (FSN) manipulation skills.

Medicine

Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model

Po-En Chiu^*1,2, Zhonghua Fu^*3,4, De-Wei Lai^5,6, Li-Wei Chou^7,8,9

¹Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, ²Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, ³Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, ⁴Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, ⁵Experimental Animal Center, Department of Molecular Biology and Cell Research, Chang Bing Show Chwan Memorial Hospital, ⁶Department of Pharmacy and Master Program, Tajen University, ⁷Department of Physical Medicine and Rehabilitation, China Medical University Hospital, ⁸Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, ⁹Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University

We present a protocol for using Fu's subcutaneous needling in a chronic constriction injury model to induce sciatic nerve pain in rats.