Gannan Normal University

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression

Ling Li¹, Mizuho Fukunaga-Kalabis¹, Meenhard Herlyn¹

¹Molecular and Cellular Oncogenesis Program, The Wistar Institute

In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.

Biology

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Deborah Mattinzoli¹, Piergiorgio Messa², Alessandro Corbelli¹, Masami Ikehata¹, Anna Mondini¹, Cristina Zennaro³, Silvia Armelloni¹, Min Li¹, Laura Giardino¹, Maria Pia Rastaldi¹

¹Renal Research Laboratory, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, ²Department of Nephrology, Dialysis and Renal Transplant, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, ³Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences, University of Trieste

Treatment of primary mouse osteoblasts with retinoic acid produces a homogeneous population of ramified cells bearing morphological and molecular features of osteocytes. The method overcomes the difficulty of obtaining and maintaining primary osteocytes in culture, and can be advantageous to study cells derived from transgenic models.

Medicine

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Ling Li¹, Klaus B. Piontek¹, Vivek Kumbhari¹, Masaharu Ishida², Florin M. Selaru¹

¹Division of Gastroenterology and Hepatology, Department of Medicine, Johns Hopkins University School of Medicine, ²Department of Surgery, Tohoku University

Bile fluid is a valuable source of extracellular vesicles/exosomes that contain potentially important biomarkers. This protocol represents a robust method to isolate exosomes from human bile for further analyses including miRNA profiling.

Biology

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Min Li^*1, Hongkun Qin^*1, Yunfei Long¹, Meiqin Cheng¹, Ling Li¹, Aijun Huang², Nian Wang³, Shuo Duan¹

¹China-USA Citrus Huanglongbing Joint Laboratory, National Navel Orange Engineering Research Center, Gannan Normal University, ²Department of Biological Sciences, Gannan Normal University, ³Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida - Institute of Food and Agricultural Sciences

In this work, a rapid, sensitive, and portable detection method for Candidatus Liberibacter asiaticus based on recombinase polymerase amplification combined with CRISPR-Cas12a was developed.

Biology

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper (Capsicum annuum L.)

Chen Cheng¹, Jianxiu Liu¹, Zhiwen Wang¹, Jiahui Liu¹, Yaqin Wang¹, Yawen Liao¹, Zhiqiang Gao¹, Zhanjun Lu¹, Bo Zhu¹, Fengxian Yao¹

¹Life Sciences College, Gannan Normal University

The paper below presents a protocol for measuring seed germination, seedling growth, and physiological indexes of two pepper varieties with salinity tolerance differences in response to six mixed salt concentrations. This protocol can be used to evaluate the salt tolerance of pepper varieties.