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Hebei University of Technology

10 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
Wei Han 1, Hui Li 1, Brahm H. Segal 2,3, Timothy S. Blackwell 1
1Department of Medicine, Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology, Roswell Park Cancer Institute, 3Department of Medicine, University at Buffalo School of Medicine

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

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Medicine

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Kasey Jividen *1, Mercedeh Javanbakht Movassagh *1, Amir Jazaeri 2, Hui Li 1
1Department of Pathology, University of Virginia, 2Department of Obstetrics & Gynecology, University of Virginia

Establishing primary endometrial stromal cell culture systems from hysterectomy specimens is a valuable biological technique and a crucial step prior to pursuing a vast array of research aims. Here, we describe two methods used to establish stromal cultures from surgically resected endometrial tissues of human patients. 

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JoVE Core

Preparation of Aligned Steel Fiber Reinforced Cementitious Composite and Its Flexural Behavior
Ru Mu 1, Luansu Wei 1, Xiaowei Wang 1, Hui Li 1, Longbang Qing 1, Jian Zhou 1, Quanming Zhao 2
1School of Civil and Transportation Engineering, Hebei University of Technology, 2School of Information Engineering, Hebei University of Technology

This protocol describes an approach for manufacturing aligned steel fiber reinforced cementitious composite by applying a uniform electromagnetic field. Aligned steel fiber reinforced cementitious composite exhibits superior mechanical properties to ordinary fiber reinforced concrete.

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Bioengineering

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Tatjana Michel 1, Antonia Link 1, Meike-Kristin Abraham 1,2, Christian Schlensak 1, Karlheinz Peter 2,3, Hans-Peter Wendel 1, Xiaowei Wang 2,3, Stefanie Krajewski 1
1Department of Thoracic and Cardiovascular Surgery, Clinical Research Laboratory, University Medical Center, 2Atherothrombosis and Vascular Biology, Baker Heart & Diabetes Institute, 3Department of Medicine, Monash University

Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA.

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Neuroscience

In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy
Amanda M Sweeney *1, Virginia Plá *1, Ting Du 1, Guojun Liu 1, Qian Sun 1, Sisi Peng 1, Benjamin A. Plog 1, Benjamin T. Kress 1, Xiaowei Wang 1, Humberto Mestre 1, Maiken Nedergaard 1,2
1Center for Translational Neuromedicine, University of Rochester Medical Center, 2Center for Translational Neuromedicine, University of Copenhagen

Transcranial optical imaging allows wide-field imaging of cerebrospinal fluid transport in the cortex of live mice through an intact skull.

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Behavior

Using a Virtual Reality Walking Simulator to Investigate Pedestrian Behavior
Hyun Chae Chung 1, Soon Ho Kim 2, Gyoojae Choi 3, Jong Won Kim 4, Moo Young Choi 2, Hui Li 1
1Department of Sport and Exercise Sciences, Kunsan National University, 2Department of Physics and Astronomy, Seoul National University, 3School of Mechanical and Automotive Engineering, Kunsan National University, 4Department of Healthcare Information Technology, Inje University

This protocol describes use of a walking simulator that serves as a safe and ecologically valid method to study pedestrian behavior in the presence of moving traffic.

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Bioengineering

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Sougata Ghosh 1,2,3, Tanay Bhagwat 3, Rohini Kitture 4, Sirikanjana Thongmee 1, Thomas J. Webster 3,5,6,7
1Department of Physics, Faculty of Science, Kasetsart University, 2Department of Microbiology, School of Science, RK. University, 3Department of Chemical Engineering, Northeastern University, 4Defence Institute of Advanced Technology Campus, Navyukti Innovations Pvt. Ltd., 5Department of Biomedical Engineering, Hebei University of Technology, 6Department of Chemistry, Saveetha School of Engineering, Institute of Medical and Technical Science, Saveetha University, 7UFPI - Universidade Federal do Piauí

Novel nanocomposites of graphene nanoribbons and hydroxyapatite nanoparticles were prepared using solution-phase synthesis. These hybrids when employed in bioactive scaffolds can exhibit potential applications in tissue engineering and bone regeneration.

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Medicine

Developing and Testing Methylated Nano-Structured Dipeptides that Inhibit Src Kinase Activity In Vitro for Anti-Cancer Applications
Alaa F. Nahhas 1, Alrayan F. Nahhas 2, Thomas J. Webster 3,4,5
1Biochemistry Department, College of Science, King Abdulaziz University, 2Independent Scholar, 3School of Health Sciences and Biomedical Engineering, Hebei University of Technology, 4School of Engineering, Saveetha University, 5Programa de Pós-graduação em Ciência e Engenharia dos Materiais, UFPI

Presented here is a protocol for designing and testing dipeptides that can inhibit Src kinase enzyme activity using acellular and cellular assays for anti-cancer applications. The peptides formulated here (W-RCH3, WCH3-RCH3, and W-R(CH3)2) inhibited Src kinase with IC50 values of 510 nM, 916 nM, and 1 µM, respectively.

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Environment

Investigating Long-Distance Transport of Perfluoroalkyl Acids in Wheat via a Split-Root Exposure Technique
Siqian Liu 1, Jian Zhou 1, Lingyan Zhu 1
1College of Natural Resources and Environment, Northwest A&F University

The present protocol describes a simple and efficient method for the long-distance transport of perfluoroalkyl acids in wheat.

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Bioengineering

High-Performance Graphene-Modified Sensing Chip for SARS-CoV-2 Detection
Parshant Kumar Sharma 1,2,3, Ebrahim Mostafavi 4,5, Nam-Young Kim 1,2,3, Thomas J. Webster 6, Ajeet Kaushik 7,8
1RFIC Bio Centre, Kwangwoon University, 2Department of Electronics Engineering, Kwangwoon University, 3NDAC Centre, Kwangwoon University, 4Stanford Cardiovascular Institute, Stanford University School of Medicine, 5Department of Medicine, Stanford University School of Medicine, 6Department of Biomedical Engineering, Hebei University of Technology, 7NanoBioTech Laboratory, Department of Environmental Engineering, Florida Polytechnic University, 8School of Engineering, University of Petroleum and Energy Studies (UPES)

The present protocol describes the fabrication of low-cost biosensing prototypes based on useful nanosystems for accurately detecting viral proteins (at the Fg level). Such a tiny sensor platform allows for point-of-care applications that can be integrated with the Internet of Medical Things (IoMT) to meet telemedicine objectives.

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