Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted QuantificationAnthony A. High 1, Haiyan Tan 1, Vishwajeeth R. Pagala 1, Mingming Niu 2,3, Ji-Hoon Cho 1, Xusheng Wang 1, Bing Bai 2, Junmin Peng 1,2
1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine
We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.
