Huaibei Normal University

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Choong Yong Ung¹, Feng Guo², Xiaoling Zhang³, Zhihui Zhu³, Shizhen Zhu^1,3

¹Department of Molecular Pharmacology & Experimental Therapeutics, Mayo Clinic College of Medicine, Center for Individualized Medicine, ²Tufts University School of Medicine, ³Department of Biochemistry and Molecular Biology, Mayo Clinic

The goal of this study is to demonstrate how the mosaic transgenesis strategy can be used in zebrafish to rapidly and efficiently assess the relative contributions of multiple oncogenes in tumor initiation and progression in vivo.

Genetics

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Yaolei Mi^1,2, Zhihui Zhu³, Guangtao Qian³, Yu Li⁴, Xiangxiao Meng¹, Jianping Xue³, Qingfu Chen⁵, Wei Sun¹, Yuhua Shi^1,2

¹Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, ²Artemisinin Research Center, China Academy of Chinese Medical Sciences, ³College of Life Science, Huaibei Normal University, ⁴Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, ⁵Research Center of Buckwheat Industry Technology, Guizhou Normal University

We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.