JoVE Logo

Sign In

Northwestern Polytechnical University

5 ARTICLES PUBLISHED IN JoVE

image

Cancer Research

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
Rui Xue Zhang 1, Tian Zhang 1, King Chen 1, Ji Cheng 1, Paris Lai 1, Andrew M. Rauth 2, K. Sandy Pang 1, Xiao Yu Wu 1
1Department of Pharmaceutical Sciences, University of Toronto, 2Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute, University Health Network

This protocol describes an efficient and convenient analytical process of sample extraction and simultaneous determination of multiple drugs, doxorubicin (DOX), mitomycin C (MMC) and a cardio-toxic DOX metabolite, doxorubicinol (DOXol), in the biological samples from a preclinical breast tumor model treated with nanoparticle formulations of synergistic drug combination.

image

Biochemistry

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
Xiaoying Qi *1, Yunyun Zhang *2, Yuan Zhang 1, Tianhui Ni 3, Wenfeng Zhang 2, Chunhua Yang 1, Jia Mi 1,4, Jiandi Zhang 2,3, Geng Tian 1
1Medicine and Pharmacy Research Center, Binzhou Medical University, 2Yantai Zestern Biotechnique Co. LTD, 3Precision Medicine research center, Binzhou Medical University, 4Department of Chemistry - BMC, Uppsala University

Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.

image

Medicine

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch
Yaguang Li 1, Junhong Liu 2,3, Gaixia Liu 1, Zhenhong Pan 2,3, Mingxia Zhang 1, Yao Ma 4, Qingxia Wei 1,5, Hongping Xia 1,6, Rui Xue Zhang 1,2, Junjun She 1
1Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, 2Institute of Medical Research, Northwestern Polytechnical University, 3School of Life Sciences, Northwestern Polytechnical University, 4Department of Medicine, Xi'an Jiaotong University, 5Princess Margaret Cancer Center, University of Health Network, 6Department of Pathology, Nanjing Medical University

The presented protocol describes a facile surgical removal of the appendix (caecal patch) in a mouse followed by the induction of inflammatory bowel disease-associated colorectal cancer. This murine appendectomy model enables investigation of the biological role of the appendix in the pathogenesis of human gastrointestinal disease.

image

Medicine

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug
Weijia Lu *1, Ruimin Miao *1, Sijun Hu 1, Junhong Liu 1, Fanqi Jin 1, Rui Xue Zhang 1
1Xi'an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University

Detailed microsurgical techniques are demonstrated to establish a longer-term jugular vein cannulation rat model for sequential blood collection in the same animal. Physiological and hematological parameters have been monitored during the rat's recovery phase. This model has been applied to study pharmacokinetics of orally administered polyphenol without inducing animal stress.

image

Immunology and Infection

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
Yang Xie *1, Yuan Zhang *2, Yitian Liu 3, Yuling Wang 4, Kun Cheng 4, Ran Zhuang 4, Ka Bian 1
1Otolaryngological Department of Tangdu Hospital, Fourth Military Medical University, 2Institute of Medical Research, Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital, Fourth Military Medical University, 4Department of Immunology, Fourth Military Medical University

Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved