Isolation of Mononuclear Cells from the Nasal Mucosa
4:52
Surface Staining for Flow Cytometry Analysis
6:08
Results: Ovalbumin-Induced Murine Model Development to Explore the Role of ILC2s in Allergic Rhinitis
7:27
Conclusion
Transcript
This protocol is established to isolate and identify the group two innate lymphoid cells from the murine nasal mucosa and explore the certain effects and underlying mechanisms of the ILC two in nasal diseases. This technique enables the researcher
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Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.