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International School for Advanced Studies

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
Anna Boccaccio 1,2, Claudia Sagheddu 1, Anna Menini 1,3
1SISSA, International School for Advanced Studies, 2Istituto di Biofisica, Consiglio Nazionale delle Ricerche, 3SISSA Unit, Italian Institute of Technology

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.

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Neuroscience

Two-Photon Polymerization 3D-Printing of Micro-scale Neuronal Cell Culture Devices
Ali Hosseini 1, Giovanni Noselli 2, Michele Giugliano 1
1Neuroscience Department, International School for Advanced Studies, 2Mathematics Department, International School for Advanced Studies

3D printing at the micrometer scale enables rapid prototyping of polymeric devices for neuronal cell cultures. As a proof-of-principle, structural connections between neurons were constrained by creating barriers and channels influencing neurite outgrowth, while the functional consequences of such manipulation were observed by extracellular electrophysiology.

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