University of Balamand

1 ARTICLES PUBLISHED IN JoVE

JoVE Core

Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations

Christina Isber¹, David L. Stockman², Ziad Daoud^2,3

¹Faculty of Medicine and Medical Sciences, University of Balamand, ²Department of Clinical Microbiology, Michigan Health Clinics, ³College of Medicine, Central Michigan University

This protocol describes how to study all possible combinations that can be obtained between four drugs in one single experiment. This method is based on the standard 96-well plate micro dilution assay and the calculation of fractional inhibitory concentrations (FICs) to evaluate the results.