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Friedrich Miescher Institute for Biomedical Research

2 ARTICLES PUBLISHED IN JoVE

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Behavior

Two-photon Calcium Imaging in Mice Navigating a Virtual Reality Environment
Marcus Leinweber *1,2, Pawel Zmarz *1,2, Peter Buchmann 3, Paul Argast 1, Mark Hübener 2, Tobias Bonhoeffer 2, Georg B. Keller 1,2
1Friedrich Miescher Institute for Biomedical Research, 2Max Planck Institute of Neurobiology, 3Department of Biosystems Science and Engineering, ETH Zurich

Here we describe the experimental procedures involved in two-photon imaging of mouse cortex during behavior in a virtual reality environment.

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Biochemistry

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
Patricia E. Nigg 1,2, Jovan Pavlovic 1
1Institute for Medical Virology, University of Zurich, 2Friedrich Miescher Institute for Biomedical Research

This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.

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