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Instituto de Investigaciones Biológicas Clemente Estable

2 ARTICLES PUBLISHED IN JoVE

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Biology

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
Rosana Rodríguez-Casuriaga 1, Gustavo A. Folle 2, Federico Santiñaque 2, Beatriz López-Carro 2, Adriana Geisinger 1,3
1Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), 2Servicio de Citometría de Flujo y Clasificación Celular (SECIF), Instituto de Investigaciones Biológicas Clemente Estable, 3Sección Bioquímica, Facultad de Ciencias, Universidad de la República

A novel protocol for the mechanical preparation of testicular cell suspensions from rodent material, avoiding enzymes and detergents, is described. The method is very simple, fast, reproducible, and renders good quality cell suspensions, which are suitable for flow sorting and RNA extraction.

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Developmental Biology

Polysome Purification from Soybean Symbiotic Nodules
María Martha Sainz 1, Carla Valeria Filippi 1, Guillermo Eastman 2,3, Mariana Sotelo-Silveira 1, C. Mauro Martinez 1, Omar Borsani 1, José Sotelo-Silveira 2,4
1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, 2Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, 3Department of Biology, University of Virginia, 4Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República

This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels.

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