JoVE Logo

Sign In

Meharry Medical College

3 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)
Donald Alcendor 1, Susan Knobel 1
1Department of Microbiology & Immunology and the Center for AIDS Health Disparities Research, Meharry Medical College

Host cell factors play a critical role in the establishment and maintenance of Kaposi's sarcoma (KS). We outline methods to identify host cell factors altered in KSHV-infected DMVEC cells, and in KS tumor tissue. Cellular genes altered by virus will serve as potential target(s) for novel therapeutics.

image

Immunology and Infection

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Muthukumar Balasubramaniam 1,2, Benem Davids 1,2, Amma B. Addai 1,2, Jui Pandhare 1,3,4, Chandravanu Dash 1,2,5
1Center for AIDS Health Disparities Research, Meharry Medical College, 2Department of Biochemistry and Cancer Biology, Meharry Medical College, 3School of Graduate Studies and Research, Meharry Medical College, 4Department of Microbiology and Immunology, Meharry Medical College, 5Tennessee Center for AIDS Research (TN-CFAR), Meharry Medical College

This report describes an in vitro assay for measuring the human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC)-associated integration activity in the cytoplasmic extracts of acutely infected cells. The integration of PIC-associated HIV-1 DNA into heterologous target DNA is quantified by using a nested real-time polymerase chain reaction strategy.

image

Genetics

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Sabyasachi Dash 1,2,3, Muthukumar Balasubramaniam 2, Chandravanu Dash 2, Jui Pandhare 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

This report describes a fast and reliable method for validating mRNA targets of cellular miRNAs. The method uses synthetic biotinylated Locked Nucleic Acid (LNA)-based miRNA mimics to capture target mRNA. Subsequently, streptavidin-coated magnetic beads are employed to pulldown the target mRNA for quantification by qPCR polymerase chain reaction.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved