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University of Hohenheim

3 ARTICLES PUBLISHED IN JoVE

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Biology

Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
Witold G. Szymanski 1, Sylwia Kierszniowska 1, Waltraud X. Schulze 1,2
1Signaling Proteomics, Max Plank Institute of Molecular Plant Physiology, 2Department of Plant Systems Biology, University of Hohenheim

Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully 15N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.

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Biology

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins
Krystina Wagner 1, Thomas K. Smylla 1, Matthias Zeger 1, Armin Huber 1
1Institute of Biology, Department of Biochemistry, University of Hohenheim

Here, non-invasive methods are described for localization of photoreceptor membrane proteins and assessment of retinal degeneration in the Drosophila compound eye using eGFP fluorescence.

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Biology

Strategies to Enhance Cultivation of Anaerobic Bacteria from Gastrointestinal Tract of Chicken
Harshita Naithani 1,2, Bibiana Rios-Galicia 1,2, Amélia Camarinha Silva 1,2, Jana Seifert 1,2
1Institute of Animal Science, University of Hohenheim, 2HoLMiR - Hohenheim Center for Livestock Microbiome Research, University of Hohenheim

The protocol presents two methodologies to improve the isolation of anaerobic intestinal bacteria. The first focuses on the isolation of a diverse range of bacteria using different culture media. The second focuses on the cultivation steps of a specific microbial group, possibly assimilating myo-inositol, to fully comprehend its ecological significance.

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