Skolkovo Institute of Science and Technology

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Mikhail Skliar^1,2, Vasiliy S. Chernyshev^3,4

¹Department of Chemical Engineering, University of Utah, ²The Nano Institute of Utah, University of Utah, ³Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, ⁴Biopharmaceutical Cluster 'Northern', Moscow Institute of Physics and Technology

A step-by-step procedure is described for label-free immobilization of exosomes and extracellular vesicles from liquid samples and their imaging by atomic force microscopy (AFM). The AFM images are used to estimate the size of the vesicles in the solution and characterize other biophysical properties.

Developmental Biology

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Rita Silvério-Alves^1,2,3, Andreia M. Gomes³, Ilia Kurochkin⁴, Kateri A. Moore^5,6, Carlos-Filipe Pereira^1,2,3

¹Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, ²Wallenberg Center for Molecular, Lund University, ³Center for Neuroscience and Cell Biology, University of Coimbra, ⁴Skolkovo Institute of Science and Technology, ⁵Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, ⁶Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

This protocol demonstrates the induction of a hemogenic program in human dermal fibroblasts by enforced expression of the transcription factors GATA2, GFI1B and FOS to generate hematopoietic stem and progenitor cells.