Methods for the Modulation and Analysis of NF-κB-dependent Adult NeurogenesisDarius Widera 1, Janine Müller 2, Yvonne Imielski 2, Peter Heimann 1, Christian Kaltschmidt 1, Barbara Kaltschmidt 2
1Cell Biology, University of Bielefeld, 2Molecular Neurobiology, University of Bielefeld
Methods for the manipulation and analysis of NF-κB-dependent adult hippocampal neurogenesis are described. A detailed protocol is presented for a dentate gyrus-dependent behavioral test (termed the spatial pattern separation-Barnes maze) for the investigation of cognitive outcome in mice. This technique should also help enable investigations in other experimental settings.
