Japan Women's University

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Yuno Natsume¹, Hsin-i Wen², Tong Zhu², Kazumi Itoh¹, Li Sheng², Kensuke Kurihara^2,3,4

¹Department of Mathematical and Physical Sciences, Faculty of Science, Japan Women's University, ²Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, ³Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science, National Institutes of Natural Sciences, ⁴Research Center for Complex Systems Biology, The University of Tokyo

Giant vesicles containing highly packed micrometer-sized components are useful cell models. The water-in-oil emulsion centrifugation method is a simple, powerful tool for the preparation of giant vesicles with encapsulated materials.

Biology

Establishment of a Clonal Culture of Unicellular Conjugating Algae

Yuki Tsuchikane¹, Takashi Hamaji², Katsumi Ota³, Syou Kato⁴

¹Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, ²Department of Botany, Graduate School of Science, Kyoto University, ³Katsumi Ota Translation Office, ⁴Wetlands International Japan

In this article, we demonstrate the establishment of clonal cultures of unicellular conjugating algal species collected from a natural field site.