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Institute for Basic Science (IBS)

7 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
Yang-Seok Park 1, Vijaya Sunkara 1, Yubin Kim 1, Won Seok Lee 1,2, Ja-Ryoung Han 1,3, Yoon-Kyoung Cho 1,4
1Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan, Republic of Korea, 2Agency for Defense Development (ADD), Daejeon, Republic of Korea, 3KOGAS (Korea Gas Corporation) Research Institute, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)

This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.

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Chemistry

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
Byung Hyo Kim 1,2, Junyoung Heo 1,2, Won Chul Lee 3, Jungwon Park 1,2
1Center for Nanoparticle Research, Institute for Basic Science (IBS), 2School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, 3Department of Mechanical Engineering, Hanyang University

Here we introduce experimental protocols for the real-time observation of a self-assembly process using liquid-cell transmission electron microscopy.

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Neuroscience

Spectral Reflectometric Microscopy on Myelinated Axons In Situ
Junhwan Kwon 1,2, Myunghwan Choi 1,2
1Department of Biomedical Engineering, Sungkyunkwan University, 2Center for Neuroscience Imaging Research, Institute for Basic Science (IBS)

Here, we present a step-by-step protocol for imaging myelinated axons in a fixed brain slice using a label-free nanoscale imaging technique based on spectral reflectometry.

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Neuroscience

µTongue: A Microfluidics-Based Functional Imaging Platform for the Tongue In Vivo
Jisoo Han 1,2, Pyonggang Choi 3, Myunghwan Choi 3
1Department of Biomedical Engineering, Sungkyunkwan University, 2Center for Neuroscience Imaging Research, Institute for Basic Science, 3School of Biological Sciences, Seoul National University

The article introduces the µTongue (microfluidics-on-a-tongue) device for functional taste cell imaging in vivo by integrating microfluidics into an intravital imaging window on the tongue.

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JoVE Journal

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
Chi-Ju Kim *1,2, Morgan D. Kuczler *1, Liang Dong 1,3, Junyoung Kim 2,4, Sarah R. Amend 1, Yoon-Kyoung Cho 2,4, Kenneth J. Pienta 1
1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)

Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.

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Biochemistry

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
Yujin Kang 1, Subin Bae 1, Soyeong An 1, Ja Yil Lee 1,2
1Department of Biological Sciences, Ulsan National Institute of Science and Technology, 2Center for Genomic Integrity, Institute for Basic Science (IBS)

DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase.

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Engineering

Fabrication of Micro-Patterned Chip with Controlled Thickness for High-Throughput Cryogenic Electron Microscopy
Min-Ho Kang 1,2, Minyoung Lee 3,4, Sungsu Kang 3,4, Jungwon Park 3,4,5,6
1Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 2Department of Biotechnology, The Catholic University of Korea, 3School of Chemical and Biological Engineering, and Institute of Chemical Processes, Seoul National University, 4Center for Nanoparticle Research, Institute of Basic Science (IBS), 5Institute of Engineering Research, College of Engineering, Seoul National University, 6Advanced Institutes of Convergence Technology, Seoul National University

A newly developed micro-patterned chip with graphene oxide windows is fabricated by applying microelectromechanical system techniques, enabling efficient and high-throughput cryogenic electron microscopy imaging of various biomolecules and nanomaterials.

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