Centro Hospitalar de Lisboa Central

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Model of Free Tissue Transfer: The Rat Epigastric Free Flap

Diogo Casal^1,4, Diogo Pais¹, Inês Iria^3,4, Eduarda Mota-Silva⁵, Maria-Angélica Almeida², Sara Alves⁶, Cláudia Pen⁶, Ana Farinho⁴, Luís Mascarenhas-Lemos^1,6, José Ferreira-Silva⁶, Mário Ferraz-Oliveira⁶, Valentina Vassilenko⁵, Paula A. Videira^3,4, João Gory O'Neill^1,5

¹Anatomy Department, NOVA Medical School, Universidade NOVA de Lisboa, ²Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central - Hospital de São José, ³UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, ⁴CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, ⁵Physics Department, Faculty of Sciences and Technology, LIBPhys, ⁶Pathology Department, Centro Hospitalar de Lisboa Central – Hospital de São José

This paper describes the steps required to raise a fasciocutaneous epigastric free flap and transfer it to the neck in the rat.

Medicine

Functional and Physiological Methods of Evaluating Median Nerve Regeneration in the Rat

Diogo Casal^*1,2, Eduarda Mota-Silva^*3, Inês Iria^4,5, Diogo Pais¹, Ana Farinho⁶, Sara Alves⁷, Cláudia Pen⁷, Luís Mascarenhas-Lemos⁷, José Ferreira-Silva⁷, Mário Ferraz-Oliveira⁷, Valentina Vassilenko³, Paula A. Videira^4,5, João Goyri-O'Neill¹

¹Anatomy Department, NOVA Medical School, ²Plastic and Reconstructive Surgery Department and Burn Unit, Centro Hospitalar de Lisboa Central, ³Physics Department, Faculty of Sciences and Technology, LIBPhys, ⁴Glycoimmunology, CEDOC, NOVA Medical School, ⁵UCIBIO, Life Sciences Department, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, ⁶Tissue Repair Unit, CEDOC, NOVA Medical School, ⁷Pathology Department, Centro Hospitalar de Lisboa Central

Presented is a protocol to produce different types of median nerve (MN) lesions and repair in the rat. Additionally, the protocol shows how to evaluate the functional recovery of the nerve using several noninvasive behavioral tests and physiological measurements.