Pharmasan Labs, inc.

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Xiaoguang Sunny Li¹, Shu Li¹, Melissa Ahrens², Gottfried Kellermann^1,2

¹Pharmasan Labs, Inc., ²NeuroScience, Inc.

A selective and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method coupled with an efficient solid phase extraction on a mixed-mode cation-exchange (MCX) 96-well microplate was developed for the measurement of free 3-nitrotyrosine (3-NT) in human urine with high throughput, which is suitable for clinical applications.